Comparative Study of Cu and Fe-Doped ZnO Nanoparticles: Synthesis, Characterization, and Multifaceted Bioactivities

Document Type : Original Article

Authors

1 Department of Physics, Faculty of Science, Malayer University, Malayer, Iran

2 Department of Physics, Faculty of Science, Razi University, Kermanshah, Iran

3 Department of Soil Science, Faculty of Agriculture, Malayer University, Malayer, Iran

4 Department of Biology, Faculty of Science, Malayer University, Malayer, Iran

Abstract

Metal oxide nanoparticles display significant roles in antimicrobial and anticancer activities. In the present study, Cu, and Fe-doped ZnO nanoparticles have been synthesized and investigated for their antioxidant, antibacterial, and anticancer properties. The above-mentioned nanoparticles have been synthesized by the low-cost and simple sol-gel method. The 2,2-Diphenyl-1-picrylhydrazyl (DPPH) assay was conducted to assess the antioxidant activity. The antibacterial activity of NPs was tested against E. coli and S. aureus bacteria according to the broth microdilution method in Mueller Hinton Broth. The anticancer potency on cancerous AsPC-1 cell lines was examined. The structural and morphology of samples confirm that all NPs formed with different crystallite sizes in the hexagonal wurtzite system. The DPPH assay showed the Zn1-XCuXO to have more antioxidant properties than other samples. We observed that the S. aureus bacterium is more sensitive to NPs than the E. coli bacterium. The strongest and weakest substances used for these bacteria are Zn1-XCuXO and Zn1-XFeXO NPs, respectively. Our anticancer results showed that the loaded drug on the NPs surfaces has more anticancer properties than pure drugs. ZnO and Zn1-XFeXO NPs possess similar anticancer properties approximately, while sunitinib@Zn1-XCuXO eliminates 92% of cancer cells at 200 μg/ml concentration. We observed that ZnO, Zn1-XFeXO, and Zn1-XCuXO NPs have antioxidant, antibacterial, and anticancer properties. Adding copper dopant to ZnO NP significantly increases its anticancer property.

Keywords

Main Subjects


[1]    Are, C., et al., Global Cancer Surgery: pragmatic solutions to improve cancer surgery outcomes worldwide. The Lancet Oncology, 2023. 24(12): p. e472-e518.
[2]    Mohamed, M., et al., A scoping review evaluating physical and cognitive functional outcomes in cancer survivors treated with chemotherapy: charting progress since the 2018 NCI think tank on cancer and aging phenotypes. Journal of Cancer Survivorship, 2024: p. 1-42.
[3]    Wang, L., et al., Radiotherapy and immunology. Journal of Experimental Medicine, 2024. 221(7): p. e20232101.
[4]    Cassinello, F., et al., Cancer surgery: how may anesthesia influence outcome? Journal of clinical anesthesia, 2015. 27(3): p. 262-272.
[5]    Grigorian, A. and C.B. O'Brien, Hepatotoxicity secondary to chemotherapy. Journal of clinical and translational hepatology, 2014. 2(2): p. 95.
[6]    Wang, S., et al., Cancer treatment-induced accelerated aging in cancer survivors: biology and assessment. Cancers, 2021. 13(3): p. 427.
[7]    Faye, G., T. Jebessa, and T. Wubalem, Biosynthesis, characterisation and antimicrobial activity of zinc oxide and nickel doped zinc oxide nanoparticles using Euphorbia abyssinica bark extract. IET nanobiotechnology, 2022. 16(1): p. 25-32.
[8]    Carofiglio, M., et al., Doped zinc oxide nanoparticles: synthesis, characterization and potential use in nanomedicine. Applied Sciences, 2020. 10(15): p. 5194.
[9]    Mehrizi, T.Z. and K.M. Hosseini, An overview on the investigation of nanomaterials' effect on plasma components: immunoglobulins and coagulation factor VIII, 2010–2020 review. Nanoscale Advances, 2021. 3(13): p. 3730-3745.
[10]  Hashmi, M.U., et al., Hydrogels incorporated with silver nanocolloids prepared from antioxidant rich Aerva javanica as disruptive agents against burn wound infections. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 2017. 529: p. 475-486.
[11]  Jiang, J., J. Pi, and J. Cai, The advancing of zinc oxide nanoparticles for biomedical applications. Bioinorganic chemistry and applications, 2018. 2018.
[12]  Vindhya, P., et al., Antimicrobial, antioxidant, cytotoxicity and photocatalytic performance of Co doped ZnO nanoparticles biosynthesized using Annona Muricata leaf extract. Journal of Environmental Health Science and Engineering, 2023. 21(1): p. 167-185.
[13]  Armelao, L., et al., Photocatalytic and antibacterial activity of TiO2 and Au/TiO2 nanosystems. Nanotechnology, 2007. 18(37): p. 375709.
[14]  Shi, L.-E., et al., Synthesis, antibacterial activity, antibacterial mechanism and food applications of ZnO nanoparticles: a review. Food Additives & Contaminants: Part A, 2014. 31(2): p. 173-186.
[15]  Sharma, S., et al., Eco-friendly Ocimum tenuiflorum green route synthesis of CuO nanoparticles: Characterizations on photocatalytic and antibacterial activities. Journal of Environmental Chemical Engineering, 2021. 9(4): p. 105395.
[16]  Wang, S., et al., Antibacterial activity of nano-SiO2 antibacterial agent grafted on wool surface. Surface and Coatings Technology, 2007. 202(3): p. 460-465.
[17]  Amininezhad, S.M., et al., The antibacterial activity of SnO2 nanoparticles against Escherichia coli and Staphylococcus aureus. Zahedan Journal of Research in Medical Sciences, 2015. 17(9).
[18]  Abinaya, S. and H.P. Kavitha, Magnesium oxide nanoparticles: effective antilarvicidal and antibacterial agents. ACS omega, 2023. 8(6): p. 5225.
[19]  Hariharan, D., et al., Enhanced photocatalysis and anticancer activity of green hydrothermal synthesized Ag@ TiO2 nanoparticles. Journal of Photochemistry and Photobiology B: Biology, 2020. 202: p. 111636.
[20]  Gebreslassie, Y.T. and F.G. Gebremeskel, Green and cost-effective biofabrication of copper oxide nanoparticles: Exploring antimicrobial and anticancer applications. Biotechnology Reports, 2024: p. e00828.
[21]  Albo Hay Allah, M.A. and H.A. Alshamsi, Green synthesis of ZnO NPs using Pontederia crassipes leaf extract: characterization, their adsorption behavior and anti-cancer property. Biomass Conversion and Biorefinery, 2024. 14(9): p. 10487-10500.
[22]  Khan, M.R., et al., Evaluation of biogenically synthesized MgO NPs anticancer activity against breast cancer cells. Saudi Journal of Biological Sciences, 2024. 31(1): p. 103874.
[23]  Moorer, W. and J. Genet, Antibacterial activity of gutta-percha cones attributed to the zinc oxide component. Oral Surgery, Oral Medicine, Oral Pathology, 1982. 53(5): p. 508-517.
[24]  Florence, A. and G. Halbert, Drug delivery and targeting. Physics in Technology, 1985. 16(4): p. 164.
[25]  Majeed, S., et al., Anticancer and apoptotic activity of biologically synthesized zinc oxide nanoparticles against human colon cancer HCT-116 cell line-in vitro study. Sustainable Chemistry and Pharmacy, 2019. 14: p. 100179.
[26]  Shochah, Q.R. and F.A. Jabir, Green synthesis of Au/ZnO nanoparticles for anticancer activity and oxidative stress against MCF-7 cell lines. Biomass Conversion and Biorefinery, 2023: p. 1-14.
[27]  Othman, M.S., et al., Antitumor activity of zinc nanoparticles synthesized with berberine on human epithelial colorectal adenocarcinoma (Caco-2) cells through acting on Cox-2/NF-kB and p53 pathways. Anti-Cancer Agents in Medicinal Chemistry (Formerly Current Medicinal Chemistry-Anti-Cancer Agents), 2022. 22(10): p. 2002-2010.
[28]  Mongy, Y. and T. Shalaby, Green synthesis of zinc oxide nanoparticles using Rhus coriaria extract and their anticancer activity against triple-negative breast cancer cells. Scientific Reports, 2024. 14(1): p. 13470.
[29]  Jain, A., R. Bhargava, and P. Poddar, Probing interaction of Gram-positive and Gram-negative bacterial cells with ZnO nanorods. Materials Science and Engineering: C, 2013. 33(3): p. 1247-1253.
[30]  Slman, A.A., Antibacterial activity of Zno nanoparticle on some gram-positive and gram-negative bacteria. Iraqi Journal of physics, 2012. 10(18): p. 5-10.
[31]  Dadi, R., et al., Antibacterial activity of ZnO and CuO nanoparticles against gram positive and gram negative strains. Materials Science and Engineering: C, 2019. 104: p. 109968.
[32]  Li, Y., C. Liao, and S.C. Tjong, Recent advances in zinc oxide nanostructures with antimicrobial activities. International Journal of Molecular Sciences, 2020. 21(22): p. 8836.
[33]  Morshed, M.N., et al., Antioxidant activity of Panax ginseng to regulate ROS in various chronic diseases. Applied Sciences, 2023. 13(5): p. 2893.
[34]  Manouchehri, I., et al., Investigation of annealing effects on optical properties of Ti thin films deposited by RF magnetron sputtering. Optik, 2016. 127(13): p. 5383-5389.
[35]  Miliauskas, G., P. Venskutonis, and T. Van Beek, Screening of radical scavenging activity of some medicinal and aromatic plant extracts. Food chemistry, 2004. 85(2): p. 231-237.
[36]  Li, H.-y., et al., Antioxidant activities of extracts and fractions from Lysimachia foenum-graecum Hance. Bioresource technology, 2009. 100(2): p. 970-974.
[37]  Fatahi Dehpahni, M., K. Chehri, and M. Azadbakht, Effect of Silver nanoparticles and l-carnitine supplement on mixed vaginitis caused by Candida albicans/Staphylococcus aureus in Mouse Models: An Experimental Study. Current Microbiology, 2021. 78: p. 3945-3956.
[38]  Dehpahni, M.F., K. Chehri, and M. Azadbakht, Therapeutic effects of silver nanoparticle and L-carnitine on aerobic vaginitis in mice: an experimental study. BioImpacts: BI, 2022. 12(1): p. 33.
[39]  Niu, J., et al., Identification and functional studies of microbial volatile organic compounds produced by Arctic flower yeasts. Frontiers in Plant Science, 2023. 13: p. 941929.
[40]  Al-Enazi, N.M., et al., In vitro anticancer and antibacterial performance of biosynthesized Ag and Ce co-doped ZnO NPs. Bioprocess and Biosystems Engineering, 2023. 46(1): p. 89-103.
[41]  Santosa, S.J., S. Sudiono, and R.S. Wibawani, Solvent-free mechanochemically synthesized Zn layered hydroxide salts for the adsorption of naphtholate AS dye. Applied Surface Science, 2020. 506: p. 144930.
[42]  Kiziltaş, H., T. Tekin, and D. Tekin, Synthesis, characterization of Fe3O4@ SiO2@ ZnO composite with a core-shell structure and evaluation of its photocatalytic activity. Journal of Environmental Chemical Engineering, 2020. 8(5): p. 104160.
[43]  Sridhar, A., et al., Dual doping effect of Ag+ & Al3+ on the structural, optical, photocatalytic properties of ZnO nanoparticles. Applied Surface Science Advances, 2023. 13: p. 100382.
[44]  Abhijith, A., A. Srivastava, and A. Srivastava. Synthesis and characterization of magnesium doped ZnO using chemical route. in Journal of Physics: Conference Series. 2020. IOP Publishing.
[45]  Xiong, G., et al., Photoluminesence and FTIR study of ZnO nanoparticles: the impurity and defect perspective. physica status solidi c, 2006. 3(10): p. 3577-3581.
[46]  Christodoulou, M.C., et al., Spectrophotometric Methods for Measurement of Antioxidant Activity in Food and Pharmaceuticals. Antioxidants, 2022. 11(11): p. 2213.
[47]  Kedare, S.B. and R. Singh, Genesis and development of DPPH method of antioxidant assay. Journal of food science and technology, 2011. 48: p. 412-422.
[48]  Lehman, K.M. and M. Grabowicz, Countering gram-negative antibiotic resistance: Recent progress in disrupting the outer membrane with novel therapeutics. Antibiotics, 2019. 8(4): p. 163.
[49]  Ebbensgaard, A., et al., The role of outer membrane proteins and lipopolysaccharides for the sensitivity of Escherichia coli to antimicrobial peptides. Frontiers in microbiology, 2018. 9: p. 2153.
[50]  Ji, P., et al., Potential of copper and copper compounds for anticancer applications. Pharmaceuticals, 2023. 16(2): p. 234.
[51]  Tang, X., et al., Copper in cancer: from limiting nutrient to therapeutic target. Frontiers in Oncology, 2023. 13: p. 1209156.
[52]  Vo, T.T.T., et al., The crosstalk between copper-induced oxidative stress and cuproptosis: a novel potential anticancer paradigm. Cell Communication and Signaling, 2024. 22(1): p. 353.